Lipidomics – The Essentials to the Cutting Edge
Day 1
8:00 AM Workshop Registration
9:00 AM – 9:10 A Welcome / Opening Remarks
All talks include ~10 minutes of discussion
SESSION 1: INTRODUCTION TO LIPIDOMICS - WHY, WHEN, AND HOW
9:10 AM – 9:30 AM Introduction to Lipidomics
9:30 AM – 10:35 AM Experimental Design
10:35 AM – 10:55 AM Coffee Break
10:55 AM – 12:00 PM Sample Preparation
12:00 PM – 1:00 P Lunch, provided by ASMS
SESSION 2: SEPARATIONS OR NOT
1:00 PM – 1:40 PM Direct Infusion/Shotgun Analyses
1:40 PM – 2:40 PM Chromatographic Separations 1 (Reverse- and Normal-Phase, HILIC)
2:40 PM – 3:15 PM Ion Mobility
3:15 PM – 3:35 PM Refreshment Break
3:35 PM – 4:30 PM Lipidomics Reporting Overview followed by problem set on experimental design
4:30 PM – 5:00 PM Round Table Discussion on Problem Set and Day 1 Recap
5:00 PM – 6:00 PM Happy Hour
Day 2
9:00 AM – 9:10 AM Day 2 Welcome
All talks include ~10 minutes of discussion
SESSION 3: IONIZATION, ION ACTIVATION AND DATA ACQUISITION
9:10 AM – 10:00 AM Ionization Techniques
10:00 AM – 10:50 AM Ion Activation Techniques
10:50 AM – 11:10 AM Coffee Break
11:10 AM – 12:00 PM Data Acquisition: Targeted, Untargeted, Data-Dependent and Data-Independent workflows
12:00 PM – 1:00 PM Lunch, provided by ASMS
SESSION 4: LIPID IDENTIFICATION AND DATA ANALYSIS
1:00 PM – 1:50 PM Lipid Identification: de novo, database searching, in silico programs
1:50 PM – 2:30 PM Data Processing: Normalization, Statistical Analyses, Relative/Absolute Abundance
2:30 PM – 2:50 PM Refreshment Break
2:50 PM – 3:30 PM Integration of Lipidomics with Pathway Analysis
3:30 PM – 4:00 PM Lipidomics Standards and Reporting Recommendations
4:00 PM – 4:45 PM Problem Set (focusing on lipid ID) and Round Table Discussion
4:45 PM – 5:00 PM Closing Remarks
5:00 PM Onward Departures at leisure